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Learn more about multiplexed imaging: CO-Detection by indEXing (CODEX) |
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Multiplexed imaging Image 40+ protein markers in tissue samplessystem composed from CODEX fluidics instrument that is connected to fluorescence KEYENCE BZ-X810 microscope. The multiplexing capability of CODEX technology is based on CODEX barcoding technology, each CODEX antibody is conjugated to a unique oligonucleotide sequence that is complementary to a unique CODEX reporter. The CODEX system can imagine a panel of ~40 antibodies on the same tissue section in one experiment. Antibodies for Human FFPE/ FF or Mouse FF samples can be bought from CODEX Akoya or manually conjugated to CODEX barcodes. The system provides single-cell resolution down to 600 nm, imaging area of 15.2X15.2 mm2, and equipped with Nikon Plan Apo Lamda 20x air objective NA 0.75 and automated, motorized stage with X, Y, Z capabilities. for more detailed information visit the CODEX site: |
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Sample preparation: Please note that the sample should be sliced on a special polylysine-coated coverglass! - contact the cell observatory unit for more info. To install the MAV plugin in Fiji follow the instructions here: https://help.codex.bio/codex/mav/installation |
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