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Learn more about multiplexed imaging: CO-Detection by indEXing (CODEX)The PhenoCycler Solution Akoya Academy Virtual Training on CODEXPhenoCycler and PhenoImager Workflows |
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Multiplexed imaging system composed from CODEX fluidics instrument that is connected to fluorescence KEYENCE BZ-X810 microscope. The multiplexing capability of CODEX technology is based on CODEX barcoding technology, each CODEX antibody is conjugated to a unique oligonucleotide sequence that is complementary to a unique CODEX reporter. The CODEX system can imagine a panel of ~40 antibodies on the same tissue section in one experiment. Antibodies for Human FFPE/ FF or Mouse FF samples can be bought from CODEX Akoya or manually conjugated to CODEX barcodes. The system provides single-cell resolution down to 600 nm, imaging area of 15.2X15.2 mm2, and equipped with Nikon Plan Apo Lamda 20x air objective NA 0.75 and automated, motorized stage with X, Y, Z capabilities. for more detailed information visit the CODEX site: |
Frequently Asked Questions:
How can I visualize the data on my pc?
download QuPath
How to add marker names to the final QPTIFF file?
Go to your data folder (D: fusion: ..) → temp folder → “marker list” → copy (Ctrl A_Ctrl C)
Open QuPath → open marker list →select all (Ctrl A) → paste (Ctrl V)
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Sample preparation: Please note that the sample should be sliced on a special polylysine-coated coverglass! - contact the cell observatory unit for more info. To install the MAV plugin in Fiji follow the instructions here: https://help.codex.bio/codex/mav/installation |
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