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Learn more about Leica SP8 2Photon:

Multiphoton Microscope Leica TCS SP8 MP

Two-Photon Microscopy

Cameras and Photosensitive Detectors I: How Do They Work?

Galvo v Resonant scanning guide

Opening .lif files in FIJI

Frequently Asked Questions:

  • How do I add and clean immersion on and off objectives correctly?

Proper Use of Objective Immersion Oil

Oiling an Objective

Determining if you’ve used the right amount of oil

Removing oil from the lens

  • How do I choose the right excitation and emission settings for my fluorophore?

Find your fluorophore on FPbase. Based on the excitation and emission data, chose the closest filter set in the software.

  • How do I save and access my data?

Save the data in:

→ open the driver data (D :) “Data_Bioimg”

→ open folder with your Weizmann user name

→ open a new folder with the date (crucial)

→ save the data (characters like ~!@#$%^&*()`;’:<>,/?[]{}  are not allowed, and files including them are not copied)

Once you are done, the computer will synchronize the correctly saved data to the “bioimg” server.

To see the data on your PC, follow the instructions in this link: BioImg Storage Server system.

**When starting to work with the Bioimaging system for the first time, please make sure you have a UNIX ID added to your WIS ID (you can check it with your unit administrator)
  • How can I visualize the data on my pc?

Download LAS X; when you install it, chose “confocal.”

You can open it in Imaris free Viewer

You can open it in ImageJ

You can open it on one of our Image Analysis Software and Workstations

  • How do I open Leica files in Fiji and Imaris?

Opening .lif files in FIJI

Follow the instructions here.

Or download macro from here.

  • Which objectives are installed in this system?

You can find the list at the end of this page, file “Objectivefinder 2P.pdf“.