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How do I add and clean immersion on and off objectives correctly?
Proper Use of Objective Immersion Oil
Determining if you’ve used the right amount of oil
How do I choose the right excitation and emission settings for my fluorophore?
Find your fluorophore on FPbase. Based on the excitation and emission data, chose the closest filter set in the software.
How do I save and access my data?
Save the data in:
→ open the driver data (D :) “Data_Bioimg”
→ open folder with your Weizmann user name
→ open a new folder with the date (crucial)
→ save the data (characters like ~!@#$%^&*()`;’:<>,/?[]{} are not allowed, and files including them are not copied)
Once you are done, the computer will synchronize the correctly saved data to the “bioimg” server.
To see the data on your PC, follow the instructions in this link: BioImg Storage Server system.
How can I visualize the data on my pc?
Download LAS X; when you install it, chose “confocal.”
You can open it in Imaris free Viewer
You can open it in ImageJ
You can open it on one of our Image Analysis Software and Workstations
How do I open Leica files in Fiji and Imaris?
Follow the instructions here.
Or download macro from here.
Which objectives are installed in this system?
You can find the list at the end of this page, file “Objectivefinder 2P.pdf“.
How do I add and clean immersion on and off objectives correctly?
Proper Use of Objective Immersion Oil
Determining if you’ve used the right amount of oil
How do I choose the right excitation and emission settings for my fluorophore?
Find your fluorophore on FPbase. Based on the excitation and emission data, chose the closest filter set in the software.
How do I save and access my data?
Save the data in:
→ open the driver data (D :) “Data_Bioimg”
→ open folder with your Weizmann user name
→ open a new folder with the date (crucial)
→ save the data (characters like ~!@#$%^&*()`;’:<>,/?[]{} are not allowed, and files including them are not copied)
Once you are done, the computer will synchronize the correctly saved data to the “bioimg” server.
To see the data on your PC, follow the instructions in this link: BioImg Storage Server system.
How can I visualize the data on my pc?
Download LAS X; when you install it, chose “confocal.”
You can open it in Imaris free Viewer
You can open it in ImageJ
You can open it on one of our Image Analysis Software and Workstations
How do I open Leica files in Fiji and Imaris?
Follow the instructions here.
Or download macro from here.
Which objectives are installed in this system?
You can find the list at the end of this page, file “Objectivefinder 2P.pdf“.
Leica TCS SP8 Confocal - MultiPhoton Suitable for cell cultures, plants, tissue sections, or thicker samples. Enables Z compensation for even illumination.
Body Configuration - Upright Lasers - UV
Visible
Multiphoton laser - Chameleon Vision II - Coherent tunable ultrafast Ti: Sapphire laser
Scanner Tandem scanner 8 kHz AOBS Detectors Reflected Internal Fully spectral
NDD detectors
Objectives
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