Adjusting the Correction Ring on the Objective - motCORR

The correction ring at the objective must be adjusted to the thickness of the specimen slide below the specimen.

Adjustment should always be made when you change the specimen carrier.

1. Start LAS X.

2. Use the objective 86x (water immersion) or the 93x (glycerol immersion).

3. Set the right imaging parameters and focus on your sample.

4. Click on “SEQ”

   Open

   

5. In the “Sequential Scan” tab that has been opened, add “SEQ” by clicking “+” and make it between stacks.

   Open

   

6. In the additional “Seq” you have opened, open one white light laser (WWL) and set it to λ=488 2%. Remove other lasers that you used in the imaging setup in this Seq.

7. Turn ON the “PMT 3:” detector and give the gain value of 500 V. Close the other detectors you used in the imaging setup in this Seq.

8. Place the detector underneath the excitation in the following manner:

9. Go to “AOBS” To reflection:

10. Select scan mode xz-y.

11. Set the format to 512 x 512.

12. Set the zoom to the smallest possible setting

13. Use bidirectional scan.

14. Click the “Live” button. A live image of the reflection line is displayed. Now you can optimize the collar ring in the “motCORR Collar Settings” tab.

15. The good setting looks like that.

16. The bad setting looks like that.

17. When you are done, click “Set”, go back to “XYZ” and set the dashed white line on the cells.

18. Remove this “Seq” by clicking “-”