The correction ring at the objective must be adjusted to the thickness of the specimen slide below the specimen.
To guarantee the correct form of the detection volume.
It would be best if you always made this adjustment when you change the specimen carrier.
You should also repeat this adjustment if the temperature changes.
Start LAS X.
Use the objective 86x or the 93x . Add the appropriate immersion to the objective.
Set the right imaging parameters and focous on your sample.
When you open the Setup Corr-ring step in the FCS Wizard, a preconfigured setting of the beam path is loaded. Adjust the settings as follows: • Select scan mode xz-y (z-Galvo). • Set the format to 512 x 512. • Set the zoom to the smallest possible setting. • Select the laser line at 488 nm. • Use bidirectional scan. • Set the AOBS to reflection. • Enable a SP PMT (no HyD and no SP FLIM PMT). • Adjust the detector range to detect the laser line as well. • Select Glow (O&U) in the color look-up table (LUT).
4. Click the Live image acquisition button. A live image of the reflection line is displayed, which you can now optimize