Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Frequently Asked Questions:

  • How do I save and access my data?

Once you saved your data at the end of the experiment, the data is synchronized to “SWAP”.

  • Why do I need to clear my sample?

In order to optimize the optical path through the sample.

  • Which clearing methods exist?

1. If it's the first time, you can start by reading the following paper:

Ariel, P. (2017). A beginner’s guide to tissue clearing. The international journal of biochemistry & cell biology84, 35-39.‏

2. Water-based clearing protocols (such as CLARITY/SWITCH), can be done in the unit, using the LifeCanvas SmartClear II.

PEGASOS method

Tip

Learn more about Light Sheet microscopy:

Light Sheet Sectioning

Richardson, D. S., & Lichtman, J. W. (2015). Clarifying tissue clearing. Cell, 162(2), 246-257.‏

Tip

Learn more about clearing methods:

  1. If it's the first time, you can start by reading the following paper: Ariel, P. (2017). A beginner’s guide to tissue clearing. The international journal of biochemistry & cell biology84, 35-39.

  2. The table that compares different methods: Richardson, D. S., & Lichtman, J. W. (2017). SnapShot: tissue clearing. Cell, 171(2), 496-496.

  3. Water-based clearing protocols (such as CLARITY/SWITCH), can be done in the unit, using the LifeCanvas SmartClear II.

  4. iDISCO method

  5. PEGASOS method

Tip

Learn more about LaVision Ultra Microscope:

Instruction Manual

UltraMicroscope II – A User Guide

...