Suitable for morphological studies of large samples (max 10*10*50 mm) cleared using water or organic solvents (RI:1.33-1.56).
Provides fast 3D imaging with a Z resolution of ~5µm.
A. Objectives:
Zoom Body (0.63 X-6.3X) WD 6mm (depends on the dipping cup). Equipped with an Olympus MVPLAPO 2X/0.5. Final magnification (1.3X – 12.6)
Infinity corrected single-lens. LVMI-fluor 4X/0.3 (Zoom optional to 8X) WD 5.7mm.
1.3 air/dipping for an overview
B. Camera:
sCMOS at 16bit
C. Excitation/Emission filters:
Excitation filter Emission Filter
470\40 525\50
514\10 595\40
545\25 595\40
560\40 630\75
640\30 690\50
710\75 810\90
Frequently Asked Questions:
How do I save and access my data?
Once you saved your data at the end of the experiment, the data is synchronized to “SWAP”.
Why do I need to clear my sample?
In order to optimize the optical path through the sample.
Learn more about Light Sheet microscopy:
Richardson, D. S., & Lichtman, J. W. (2015). Clarifying tissue clearing. Cell, 162(2), 246-257.
Learn more about clearing methods:
If it's the first time, you can start by reading the following paper: Ariel, P. (2017). A beginner’s guide to tissue clearing. The international journal of biochemistry & cell biology, 84, 35-39.
The table that compares different methods: Richardson, D. S., & Lichtman, J. W. (2017). SnapShot: tissue clearing. Cell, 171(2), 496-496.
Water-based clearing protocols (such as CLARITY/SWITCH), can be done in the unit, using the LifeCanvas SmartClear II.
