Dragonfly 500 spinning disk

Frequently Asked Questions:

  • How do I add and clean immersion on and off objectives correctly?

Proper Use of Objective Immersion Oil

Oiling an Objective

Determining if you’ve used the right amount of oil

Removing oil from the lens

  • How do I choose the right excitation and emission settings for my fluorophore?

Find your fluorophore on FPbase. Based on the excitation and emission data, chose the closest filter set in the software.

  • How do I save and access my data?

Once you are done with the imaging, the data is automatically saved on the driver ImageDisk (D :) “FusionImages” folder with the current date.

First, discard the images you don't need and leave the images you want to keep.

Then, copy these images to the bioimg server in the following way:

→ open the driver ImageDisk (D :) “Data_Bioimg”

→ open folder with your Weizmann user name

→ open a new folder with the date (crucial)

→ transfer the data (characters like ~!@#$%^&*()`;’:<>,/?[]{}  are not allowed, and files including them are not copied)

Once you are done, the computer will synchronize the correctly saved data to the “bioimg” server.

To see the data on your PC, follow the instructions in this link: BioImg Storage Server system.

**When starting to work with the Bioimaging system for the first time, please make sure you have a UNIX ID added to your WIS ID (you can check it with your unit administrator)
  • How can I visualize the data on my pc?

You can open it in ImageJ

You can open it in Imaris free Viewer

You can open it on one of our Image Analysis Software and Workstations

  • Which objectives are installed in this system?

You can find the list at the end of this page, file “Objectivefinder_spinning disk.pdf“.

Offers fast and sensitive 3D imaging, utilizing WF imaging, confocal imaging with 2 different pinhole sizes (40µm for higher contrast/25 µm for higher resolution), or TIRF (total internal reflection) imaging.

  • Excitation wavelengths (nm):

405-445-488-514-561-633

  • Emission filters (nm):

450-525-600-647

*dichroic mirror deflects between GFP-RFP/CFP-YFP detection

Objectives:

  1. 5x/0.15 air

  2. 10X/0.1 air

  3. 25X/0.95 w

  4. 40X/1.1 w

  5. 63X/1.3 G

  6. 63X/1.4 O

  7. 100X/1.47 TIRF

  • Multi-color fast acquisition as well as simultaneous dual-color imaging for all the above modes

  • Finite-burst experiment design allows 2D dual-color simultaneous imaging at 100 fps (2048*2048 format)

  • Enables fast, accurate navigation within large areas (16*14mm)

  •  2 Zyla 4.2 camera with flexible AOI (area of interest) definition, binning (for enhanced sensitivity), and zoom options between 1X-1.5X-2X

  • Mosaic for simultaneous, accurate illumination (photobleaching/photoactivation) of multiple areas on the sample

  • Environmental control and auto-focus for live experiments, stage holder fitted to 35mm dishes, multi-well plates, and slides

  • Fusion software offers GPU-enhanced deconvolution and instant Imaris view

  File Modified

JPEG File 20210125_084012.jpg

Jan 25, 2021 by Inna Goliand

PDF File Objectivefinder_spinning disk.pdf

May 09, 2021 by Inna Goliand